Instrumentation

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• Applied Biosystems 2 x 96 well MiniAmp Instruments
• Applied Biosystems QuantStudio 5 Real-Time PCR (384-well)
• Applied Biosystems QuantStudio 3 Real-Time PCR (96-well)
• Cole-Parmer Techne PrimePro48 Real Time qPCR
• Qiagen QIAcuity Digital PCR System (4x96-well throughput)
  • The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more.
• Thermofisher ProFlex (7x96 instruments); 1xdual-head 384 instrument; 1xtriple-head 32 instrument)

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• Integra VIAFLO96 semi-automated pipettor
• Integra Assist Plus pipetting robot
• Integra Mini 96 pipettor
• OpenTrons OT2 Lab Robot (x3)
  • The OT-2 lab robot is an open-source user friendly liquid handling robot to automate tasks such as library prep, ribosomal RNA depletion, poly(A) mRNA enrichment, sample normalization and re-pooling of sequencing libraries.
• Revvity Sciclone® G3 NGSx Workstation
  • The Sciclone G3 workstation is a fully automated, high-throughput NGS library preparation system that can process up to 96 samples at a time for DNA and RNA libraries, including standard RNAseq, rRNA depletion, DNAseq, microRNAseq, methyl-seq, hybridization capture, and more. Full automation allows for high reproducibility and rapid turnaround.
• Revvity JANUS
  • The Janus is a workstation used for automation of pre-processing of air filter samples for downstream nucleic acid extraction.
• Revvity BioQule
  • The BioQule is a fully automated, low-throughput NGS library preparation system that can process up to 8 samples at a time for DNA and RNA libraries. Eventually, the BioQule system is expected to be able to integrate DNA extraction and DNA library preparation into a single automated workflow with minimal hands-on effort required.

• 10X CytAssist
  • shared with Rush University Histology Core Facility
• Fluent Biosciences
  • Cell separation system

The GMCF facility houses several small-to-medium sized sequencers, with big data generation performed externally on Illumina NovaSeq6000 instruments (NovaSeq6000 X coming soon) and on PacBio Sequel II devices. Internal instruments are used for NGS amplicon sequencing and projects with small data needs or those where a short turn-around-time is essential. In addition, most Illumina libraries made at the GMCF facility are initially sequenced on a small sequencing run (‘QC sequencing’) to evaluate library quality and to standardize sequencing depth between samples.

• 2D barcode Matrix system, including:
  • VisionMate High Speed Barcode Reader
  • VisionMate Wireless Reader (x2)